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By default IIS is not enabled in the stadard vista install. It therefore first needs to be turned on.

The following link provides a short cut to the MS knowledge base article on setting up Vista IIS or web application folders

http://msdn.microsoft.com/en-us/library/aa964620.aspx

For the sake of completeness here is the link for 2003 server

http://www.microsoft.com/technet/prodtechnol/WindowsServer2003/Library/IIS/7ecaa5f3-5499-4887-8c9d-00aba71125df.mspx?mfr=true

Also the following article may be of assistance.

http://www.windowsnetworking.com/articles_tutorials/Web-Sites-Windows-2003.html

Trouble shooting these permissions can be greatly simplified by using the Process Monitor (Sysinternals) available here:

http://technet.microsoft.com/en-us/sysinternals/bb896645.aspx

There is also a good article on its use here:

http://blogs.msdn.com/b/webtopics/archive/2009/06/25/troubleshooting-http-401-3-errors-with-process-monitor.aspx

The folowing article expains access control:

http://www.microsoft.com/technet/prodtechnol/WindowsServer2003/Library/IIS/66c1550e-9cd7-4891-a706-ddce88494169.mspx?mfr=true

If you get a 401.3 error when you try to browse the new site through IIS then

http://www.microsoft.com/technet/prodtechnol/WindowsServer2003/Library/IIS/66c1550e-9cd7-4891-a706-ddce88494169.mspx?mfr=true